The prevalence of the tattooed population in Europe rises continuously, and so does the diversity of used inks. The entry into force of the European harmonised restrictions of substances used in tattoo inks and permanent make-up (PMU) spreads a new light on consumer safety. While progress is made with regard to the identification of health risks associated with tattoos, many adverse conditions such as systemic sensitisation or toxicity of degradation products remain unresolved. The 2nd International Conference on Tattoo Safety in Berlin brings together experts in the fields of toxicology, legislation, and manufacturing. The main objective of the conference is to set a frame in which the requirements for the toxicological evaluation of tattoo inks as well as their manufacturing quality shall be implemented. This shall be achieved by identification of information gaps in terms of toxicological evaluation and practical approaches for their compensation. The recent research insights will be evaluated from a toxicological perspective. Furthermore, progress made recently with respect to the regulation of tattoos and PMU under REACH will be discussed. The voice of the practitioners and manufacturers will be heard to understand the needs of the industry as well as the compliance challenges.